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anti mouse cd206 antibody  (Elabscience Biotechnology)


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    Elabscience Biotechnology anti mouse cd206 antibody
    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of <t>CD206</t> (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
    Anti Mouse Cd206 Antibody, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd206+antibody/PE+Anti-Mouse+CD206%2FMMR+Antibody/pmc13091142-309-8-11
    Average 95 stars, based on 59 article reviews
    anti mouse cd206 antibody - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration"

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2026.04.004

    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
    Figure Legend Snippet: LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Techniques Used: Activation Assay, Flow Cytometry, RNA Sequencing, Marker, Expressing, Injection, Immunofluorescence, Co-Culture Assay, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay

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    Incubation:

    Article Title: Matrix mechanical remodeled carrier-free nanosystem for programmable closed-loop reversal of liver fibrosis via STING alkylation
    Article Snippet: .. The cells were then incubated with PE-conjugated anti-mouse CD86 antibody (Elabscience) and FITC-conjugated anti-mouse CD206 antibody (Elabscience) for 30 min. Last, the cells were washed with PBS and resuspended in 500 μl of PBS, and the fluorescence intensity was detected using flow cytometry (Beckman, CytoFLEX, USA). ..

    Article Title: O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: In vivo and in vitro study
    Article Snippet: .. The cells were then washed again with PBS and incubated with phycoerythrin-conjugated anti-mouse CD86 antibody (E-AB-F0994D; Elabscience, Wuhan, China) and allophycocyanin-conjugated anti-mouse CD206 antibody (E-AB-F1135E; Elabscience) at 4 °C for 30 minutes. .. Following incubation, the cells were resuspended in PBS, and the expression of surface markers was detected using a CytoFLEX flow cytometer (Beckman Coulter, Fullerton, CA, United States).

    Article Title: Matrix mechanical remodeled carrier-free nanosystem for programmable closed-loop reversal of liver fibrosis via STING alkylation
    Article Snippet: .. After that, the supernatant was discarded, and the cells were incubated in the medium containing phycoerythrin (PE)–conjugated anti-mouse CD86 antibody (Elabscience) and fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD206 antibody (Elabscience) at 4°C for 30 min. .. Nuclei were stained with Hoechst 33342 at room temperature for 15 min, and then the cells were washed several times using PBS buffer solution and imaged under a CLSM (Nikon A1R+, Shanghai, China).

    Fluorescence:

    Article Title: Matrix mechanical remodeled carrier-free nanosystem for programmable closed-loop reversal of liver fibrosis via STING alkylation
    Article Snippet: .. The cells were then incubated with PE-conjugated anti-mouse CD86 antibody (Elabscience) and FITC-conjugated anti-mouse CD206 antibody (Elabscience) for 30 min. Last, the cells were washed with PBS and resuspended in 500 μl of PBS, and the fluorescence intensity was detected using flow cytometry (Beckman, CytoFLEX, USA). ..

    Flow Cytometry:

    Article Title: Matrix mechanical remodeled carrier-free nanosystem for programmable closed-loop reversal of liver fibrosis via STING alkylation
    Article Snippet: .. The cells were then incubated with PE-conjugated anti-mouse CD86 antibody (Elabscience) and FITC-conjugated anti-mouse CD206 antibody (Elabscience) for 30 min. Last, the cells were washed with PBS and resuspended in 500 μl of PBS, and the fluorescence intensity was detected using flow cytometry (Beckman, CytoFLEX, USA). ..

    Labeling:

    Article Title: Spin-mediated and proton-coupled electron transfer boost NADH oxidase-like activity of dual-atom metallozymes
    Article Snippet: Nicotinamide adenine dinucleotide (NAD) as a pivotal coenzyme plays a central role in the pathogenesis of various inflammatory diseases.. However, both natural and artificial NADH oxidase (NOX) systems exhibit limited efficacy in NAD regeneration.. Here, we developed a RuCo heterogeneous dual atom metallozymes (DAMs) with over-natural NOX activity through spin-mediated and proton-coupled electron transfer (PCET).

    Staining:

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration
    Article Snippet: .. Following permeabilization, cells were stained with Phycoerythrin (PE)-conjugated anti-mouse CD206 antibody (Elabscience, E-AB-F1135D) for 30 min at 4 °C in the dark. .. After staining, cells were washed twice, resuspended in PBS, and analyzed on a flow cytometer (BD LSRFortessa).

    Article Title: Synergistic optimization of mechanical properties and biocompatibility in microwave-sintered graphene oxide/SrO-Cr2O3-ZTA composite ceramics for artificial joints
    Article Snippet: The development of materials for artificial joint replacements requires the coordinated optimization of mechanical strength, wear resistance, and biocompatibility to ensure long-term implant performance.. Zirconiatoughened alumina (ZTA) ceramics have been widely used in joint replacements because of their excellent bioinertness and moderate toughness.. However, their relatively low flexural strength, limited fracture toughness, and suboptimal tribological properties restrict their long-term reliability and durability under dynamic physiological loading conditions.

    other:

    Article Title: Gegen Qinlian decoction remodels tumor immune microenvironment and inhibits aerobic glycolysis with the synergistic combination of CPT-11 chemotherapy in colorectal cancer therapy.
    Article Snippet: Ethnopharmacological relevance: Although several traditional Chinese medicine formulas have demonstrated remarkable outcomes in suppressing the severe gastrointestinal toxicity induced by irinotecan (CPT-11), few studies have investigated whether enhanced anti-cancer efficacy and reduced intestinal toxicity can be achieved through co-administration.. CPT-11, as a first-line drug for treating colorectal cancer, has the side effect of intestinal toxicity.. Previous studies have primarily focused on using traditional Chinese medicine to alleviate diarrhea caused by CPT-11.

    Microscopy:

    Article Title: Synergistic optimization of mechanical properties and biocompatibility in microwave-sintered graphene oxide/SrO-Cr2O3-ZTA composite ceramics for artificial joints
    Article Snippet: The development of materials for artificial joint replacements requires the coordinated optimization of mechanical strength, wear resistance, and biocompatibility to ensure long-term implant performance.. Zirconiatoughened alumina (ZTA) ceramics have been widely used in joint replacements because of their excellent bioinertness and moderate toughness.. However, their relatively low flexural strength, limited fracture toughness, and suboptimal tribological properties restrict their long-term reliability and durability under dynamic physiological loading conditions.

    Colorimetric Assay:

    Article Title: Synergistic optimization of mechanical properties and biocompatibility in microwave-sintered graphene oxide/SrO-Cr2O3-ZTA composite ceramics for artificial joints
    Article Snippet: The development of materials for artificial joint replacements requires the coordinated optimization of mechanical strength, wear resistance, and biocompatibility to ensure long-term implant performance.. Zirconiatoughened alumina (ZTA) ceramics have been widely used in joint replacements because of their excellent bioinertness and moderate toughness.. However, their relatively low flexural strength, limited fracture toughness, and suboptimal tribological properties restrict their long-term reliability and durability under dynamic physiological loading conditions.



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    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of <t>CD206</t> (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.
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    Image Search Results


    LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Journal: Bioactive Materials

    Article Title: Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regeneration

    doi: 10.1016/j.bioactmat.2026.04.004

    Figure Lengend Snippet: LA released from T-gel degradation induces macrophage M2 polarization and promotes fibroblast activation and collagen production. (A) Flow cytometry analysis of the association between T-gel degradation products and macrophage M2 polarization ( n = 3, data represent mean ± s.d.). ∗∗∗∗P < 0.0001. (B) RNA-seq analysis of M2 macrophage marker expression in tissues one week after ID and SC injection of T-gel. (C–D) Immunofluorescence analysis of CD206 (C) and FAPα (D) expression in tissues ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (E) Schematic diagram of the co-culture system showing RAW264.7 macrophages pretreated with T-gel extract medium that were subsequently co-cultured with L929 fibroblasts. (F–G) Collagen expression in L929 cells from the co-culture system assessed by Western blot (F) and ELISA (G). “C” represents RAW264.7 pretreated with blank medium; “T” represents RAW264.7 pretreated with T-gel extract medium. n = 3, data represent mean ± s.d. ∗P < 0.05 and ∗∗P < 0.01. (H) ELISA analysis of TGFβ expression in RAW264.7 cells treated with T-gel extract medium ( n = 3, data represent mean ± s.d.). ∗∗P < 0.01. (I) Schematic representation of LA, released during T-gel degradation, programming macrophages toward an M2 phenotype and subsequently promoting fibroblast activation and collagen secretion.

    Article Snippet: Following permeabilization, cells were stained with Phycoerythrin (PE)-conjugated anti-mouse CD206 antibody (Elabscience, E-AB-F1135D) for 30 min at 4 °C in the dark.

    Techniques: Activation Assay, Flow Cytometry, RNA Sequencing, Marker, Expressing, Injection, Immunofluorescence, Co-Culture Assay, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay

    A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Journal: bioRxiv

    Article Title: Aspirin hastens resolution of skeletal muscle inflammation and promotes recovery of muscle strength following acute injury

    doi: 10.64898/2026.04.21.719989

    Figure Lengend Snippet: A: Representative histological (H & E) and immunofluorescence images of TA muscle cross-sections at day 1 post-injury following treatment with vehicle (VEH), indomethacin (INDO), aspirin (ASA), or a combination of INDO + ASA. Sections are stained for Ly6G (neutrophils, red), CD68 (total macrophages, green), CD206 (M2-like macrophages, red), with DAPI (nuclei, blue) and Laminin (LAM, white) to visualize fiber boundaries. B-E : Quantification of day 1 post-injury inflammatory markers including Ly6G + cell density ( B ), CD68 + cell density ( C ), the ratio of Ly6G + to total CD68 + cells ( D ), and the M1/M2 macrophage ratio ( E ). F: Representative H & E and immunofluorescence images at day 3 post-injury for the same treatment groups and markers. G-J: Quantitative analysis of inflammatory cell dynamics at day 3 post-injury, including Ly6G + cell density ( G ), CD68 + cell area as a percentage of total tissue ( H ), CD206 + cell densities ( I ), and the M1/M2 ratio ( J ). All data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Holm-Šídák post hoc tests. Groups labeled with different letters are significantly different from one another, while groups sharing a common letter are not significantly different.

    Article Snippet: Primary antibodies used include MyHC type I [Developmental Studies Hybridoma Bank (DSHB), BA-D5c, 1:100], MyHC type IIA (DSHB, SC-71c, 1:100), MyHC type IIB (DSHB, BF-F3c, 1:100), eMHC (DSHB, F1.652s, 1:20), Ly6G (GR1) (Bio-Rad, MCA2387, 1:50), CD68 (Bio-Rad, MCA1957, 1:200), CD206 (Bio-Rad, MCA2387, 1:50), and laminin (Abcam, ab7463, 1:200).

    Techniques: Immunofluorescence, Staining, Labeling